hoechst live cell staining solution Search Results


99
Beyotime hoechst live cell staining solution
Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI <t>double</t> <t>staining.</t> The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by <t>Hoechst</t> to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.
Hoechst Live Cell Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+live+cell+staining+solution/pmc08046541-66-6-11?v=Beyotime
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hoechst live cell staining solution - by Bioz Stars, 2026-07
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90
Cayman Chemical live/dead cell assay staining with hoechst 33342 cat no. 875756-97-1
Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI <t>double</t> <t>staining.</t> The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by <t>Hoechst</t> to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.
Live/Dead Cell Assay Staining With Hoechst 33342 Cat No. 875756 97 1, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+live+cell+staining+solution/pm32245975-100-7-9?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
live/dead cell assay staining with hoechst 33342 cat no. 875756-97-1 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

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Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI double staining. The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by Hoechst to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway

doi: 10.1155/2021/6623464

Figure Lengend Snippet: Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI double staining. The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by Hoechst to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.

Article Snippet: For Hoechst staining, 10 μ L Hoechst live cell staining solution (Beyotime Institute of Biotechnology) was added to the culture medium and mixed gently.

Techniques: Flow Cytometry, Double Staining, Control, Staining, Western Blot